A buffer salt used mainly in electrophoresis and chromatography
Tris acetate salt is frequently used in the preparation of TAE
(Tris-acetate-EDTA) buffer, which is used as a running buffer and
in agarose gels. Tris Acetate-EDTA buffer is used for DNA agarose
gel electrophoresis but is also used for non-denaturing RNA agarose
gel electrophoresis. Double-stranded DNA tends to run faster in TAE
than in other buffers and may become exhausted during extended
electrophoresis. Buffer circulation or buffer replacement during
extended electrophoresis can remedy the lower buffering capacity.
May be used at various concentrations to study the mobility of DNA
in solution. Since borate in TBE buffer (Tris-Borate-EDTA Buffer,
10X Powder Pack, is a strong inhibitor for many enzymes, TAE buffer
is recommended when looking at enzymatic applications for the DNA
sample. Tris acetate salt is also a buffer with high sensitivity in
ATP assays with firefly luciferase.